本文已被：浏览 1140次 下载 377次
投稿时间：2019-06-17
投稿时间：2019-06-17
中文摘要: 以山竹果皮为原料，采用超声波辅助水提法提取其中的多糖。单因素试验证实了各因素影响多糖提取效果的显著程度，在此基础上利用响应面法对提取工艺进行优化，获得最佳工艺参数：液料比49∶1（mL/g）、提取温度46℃、超声时间26 min、超声功率240 W。在此条件下测得多糖提取率为（6.51±0.17）%，与模型预测值6.72%相当。采用体外测试法考察山竹果皮多糖的抗氧化活性。当多糖浓度为1.0 mg/mL时，对羟基自由基（·OH）、超氧阴离子自由基（·O2-）、DPPH自由基的清除率分别可达79.06%、81.57%、79.23%，总还原力达到0.698，且其性能随多糖浓度的增加而增强，说明山竹果皮多糖具有较好的抗氧化活性。
Abstract:Mangosteen peels as starting materials， studies on ultrasound-assisted extraction of polysaccharides were carried out.Based on results of single factored experiments，the extraction process was optimized by response surface methodology，and the best extraction conditions were obtained：liquid-material ratio 49∶1（mL/g），temperature 46℃，ultrasonic time 26 min，and ultrasonic power 240 W，which resulted in the yield of （6.51±0.17）%similar to the expected yield 6.72%of the regression model.Investigations of antioxidant activity revealed that polysaccharides in mangosteen peels had good scavenging activities on radicals.The clearance rates on hydroxyl radical（·OH），superoxide anion radical（·O2-），DPPH radical were 79.06%，81.57%，79.23%respectively when the concentration of polysaccharides reached 1.0 mg/mL，and the total reducing power was 0.698，which were more strong with higher concentration.
keywords: polysaccharides mangosteen peels ultrasound-assisted extracted response surface methodology antioxidant activity
文章编号：202010018 中图分类号： 文献标志码：
基金项目:2017年广西高校中青年教师基础能力提升项目（2017KY0719）；2017年度广西自然科学基金项目（2017GXNSFBA198225）
引用文本：
津ICP备20000603号-1