Abstract:The preliminary study on the degradation mechanism of Morchella species was carried out.Degraded Morchella material was obtained by several times of normal Morchella species，and normal hyphae and degraded hyphae were treated with specific staining reagents respectively to observe the ability of hyphae to scavenge reactive oxygen species and mitochondrial membrane，changes in potential levels and changes in mitochondrial morphology explored the causes of bacterial degradation.The transferred Morchella was obviously degraded.The normal hyphae showed light blue-violet under the light microscope after nitro blue tetrazolium（NBT）staining.The degraded hyphae formed a blue-violet substance after a period of time；the normal hyphae passed the fluorescent dye Mito-Tracker Red CMXRos stained with red fluorescence under fluorescent microscope，while degenerating hyphae showed only weak red fluorescence.The normal mycelia stained with the fluorescent dye Mito-Tracker Green had bright mitochondrial spots，but the degenerated mycelia did not.This experiment preliminarily clarified the mechanism of hyphae degradation of Morchella，and provided a theoretical basis for the study of Morchella hyphae degradation.