Abstract:Milk is one of the earliest foods introduced into infant diets and one of the most common causes of food allergies.Therefore，the development of hypoallergenic milk powder is of great significance.Four-weekold female BALB/c mice were sensitized by gavage.The sensitization of partially hydrolyzed whey protein infant formula was evaluated by observing the allergic symptoms，calculating the spleen index and measuring the contents of total immunoglobulin E （IgE）and histamine in serum.The results showed that the whey protein concentrate group had obvious allergy symptoms，and the contents of serum total immunoglobulin E（IgE），histamine and spleen index were significantly higher than the blank and adjuvant group，indicating that the milk protein sensitization model was successfully established.There were no allergic symptoms in the partially hydrolyzed whey protein infant formula group.Compared with the blank group and the adjuvant group，there were no significant differences in the contents of serum total immunoglobulin E （IgE），histamine and spleen index，indicating that the partially hydrolyzed whey protein infant formula did not cause allergy in mice.Similarly，the whey protein hydrolysate did not cause sensitization in mice due to the destruction of whey protein antigen epitope by deep hydrolysis.However，the contents of serum total immunoglobulin E （IgE），histamine and spleen index in the mice sensitized by skim milk powder were significantly higher than those in the blank group and the adjuvant group，indicating that skim milk powder also caused sensitization in mice.Therefore，partially hydrolyzed whey protein infant formula could effectively reduce or alleviate milk allergy symptoms，but it still needed to be verified by more clinical trials.