Abstract:The methylglyoxal-modified β-lactoglobulin product（MG-bLG）was used as a representative of the protein-bound advanced glycation end products （AGEs）to study the effect of protein-bound AGEs on insulin resistance（IR）in mice by intragastric ingestion.After long-term intake of MG-bLG，compared with the control group（group C），the experimental group（MG-bLG group）increased the food intake and weight，the content of serum low-density lipoprotein，total cholesterol and triglycerides were significantly increased （P<0.05），the fasting blood glucose was significantly increased to 7.1 mmol/L （P<0.05），the fasting insulin level was significantly increased to 14.8 mU/L （P<0.05），homeostasis model assessment-insulin resistance index was significantly increased （P<0.05），homeostasis model assessment-insulin sensitivity index was significantly decreased （P<0.05）.In addition，the serum AGEs levels in the MG-bLG group were significantly increased to 131.99 pg/mL （P<0.05）.Therefore，long-term intake of AGEs can change the glucose-lipid metabolism，promote the formation of insulin resistance，and increase the risk of type 2 diabetes in the body.