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投稿时间：2019-05-31
投稿时间：2019-05-31
中文摘要: 以蛹虫草多糖（Cordyceps militaris polysaccharides，CMP）的提取得率为指标，通过单因素和响应面法对CMP的提取工艺进行优化。采用乙醇分级法，将CMP 进行乙醇分级，分别得到4 种多糖组分（CMP20、CMP40、CMP60 和CMP80），并对不同多糖的得率、组分含量及抗氧化活性进行比较。结果表明，CMP 的最优提取条件为温度84 ℃、液料比 33 ∶1（mL/g）和时间 128 min。在此条件下，实际提取得率为 7.83%；CMP20、CMP40、CMP60 和 CMP80 的得率分别为7.06%、15.07%、17.83%、25.23%。其中，CMP80 的得率最高，蛋白含量最低，仅为1.47%；5 种多糖均具有一定的抗氧化活性，CMP60 的还原力和DPPH 自由基清除率均为最高，CMP80 的羟自由基的清除率最高。
Abstract:The extraction process of Cordyceps militaris polysaccharides （CMP）was optimized by single factor and response surface methodology.CMP20，CMP40，CMP60 and CMP80 were fractionated from CMP by alcohol fractional precipitation technique.And the extraction yields，components content and antioxidant activities of the different polysaccharides were compared.The results showed that the optimal extraction process for CMP were as follows：extraction temperature 84 ℃，the liquid to raw material 33 ∶1（mL/g），extraction time 128 min.Under these conditions，the extraction yield was 7.83 %.The extraction ratios of CMP20，CMP40，CMP60 and CMP80 were 7.06 %，15.07 %，17.83 % and 25.23 %，respectively.CMP80 had the highest extraction ratio and the lowest protein content only 1.47%.The five polysaccharides had antioxidant activities，the reducing power and DPPH radicals scavenging rates of CMP60 were the highest，and the hydroxyl radicals scavenging rates of CMP80 was the highest among them.
keywords: Cordyceps militaris polysaccharides response surface methodology ethanol fractional precipitation antioxidant activity
文章编号：202009017 中图分类号： 文献标志码：
基金项目:聊城大学科研基金项目（318011707）
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