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食品研究与开发:2020,41(9):61-69
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超声波辅助提取对花生蛋白结构与功能特性的影响
(1.河南工业大学粮油食品学院,河南郑州450001;2.河南省南街村(集团)有限公司,河南漯河462000)
Effect of Ultrasound-assisted Extraction on the Structure and Functional Properties of Peanut Protein
(1.College of Food Science and Technology,Henan University of Technology,Zhengzhou 450001,Henan,China;2.Henan Nanjiecun(Group)Co.,Ltd.,Luohe 462000,Henan,China)
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投稿时间:2019-05-31    
中文摘要: 该文研究超声波参量对花生蛋白的溶解度、持水性、持油性及起泡性等功能特性的影响,同时借助聚丙烯酰胺凝胶电泳、红外光谱等方法对花生蛋白的结构进行表征。结果表明,在超声波功率密度3.17 W/cm3、时间30 min、温度35 ℃和占空比50%时花生蛋白的溶解度、持水性和起泡性等功能特性有所改善;同时对花生蛋白的结构分析结果表明,超声波辅助提取的花生蛋白α-螺旋含量减少,β-转角含量增加,且花生蛋白分别在66.2 kDa 和14.4 kDa 处生成新条带。
中文关键词: 超声波  辅助提取  花生蛋白  功能特性  结构
Abstract:Effects of ultrasound parameters on peanut protein solubility,water-holding,oil-holding,and foaming ability were investigated.The structure properties of peanut protein were also studied by SDS-PAGE,and infrared spectroscopy(FT-IR).The results indicated that the solubility,water-holding foaming ability and other functional properties of peanut protein were improved when the ultrasonic power density was 3.17 W/cm3,time 30 min,temperature 35 ℃ and duty cycle 50 %.According to structure analysis,α-helix content of ultrasound -assisted extraction peanut protein decreased while β -turn content increased.New bands of ultrasound-assisted extraction peanut protein were observed at 66.2 kDa and 14.4 kDa,respectively.
文章编号:202009011     中图分类号:    文献标志码:
基金项目:河南省重点研发与推广专项(182102110253)
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