Abstract:The effect of inoculum amount，initial sugar content，white mulberry and lemon juice ratio，initial pH value，initial SO2 concentration and fermentation temperature were studied through single-factor experiment and L9（33）orthogonal test.The optimal parameters for this method were that the initial sugar content of 24%，the initial pH of 3.6 and fermentation temperature of 25 ℃.The wine obtained under the condition of the brewing was clarification brilliantly，coordination of fragrance，taste mellow long，sensory score of 89.7 points.