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投稿时间：2020-01-31
投稿时间：2020-01-31
中文摘要: 以甜瓜籽为原料，水解度为考察指标，采用胰蛋白酶和碱性蛋白酶双酶协同酶解法制备甜瓜籽多肽。通过单因素试验和响应面设计试验对酶解条件进行优化，结果表明：酶解的最佳工艺条件为底物浓度为3 %、加酶量为4 %、酶解时间为180 min、酶解温度为50 ℃、pH 值为8、双酶质量比为6∶4，水解度达到最大值13.85%。
Abstract:Melon seeds was used as raw materials，and taking the degree of hydrolysis as an index，trypsin and alkaline protease were used to prepare the melon seed polypeptide. The single-factor test and response surface design test were used to optimize the enzymolysis conditions. The results showed that the best conditions for enzymolysis were found to be：substrate concentration 3 %；enzyme amount 4 %；hydrolysis time 180 min；hydrolysis temperature 50 ℃；pH 8 and dual-enzymatic mass ratio 6∶4. The degree of hydrolysis reached a maximum of 13.85%.
keywords: melon seed polypeptide dual-enzymatic hydrolysis the degree of hydrolysis process optimization
文章编号：202008015 中图分类号： 文献标志码：
基金项目:长春大学与吉林大学联合培养“协同培养”项目
| Author Name | Affiliation |
| WANG Si-qi，FU Jing-yi，DING Xiu-qing，LIU Jia-xin，ZHAO Jun | College of Food Science and Engineering，Changchun University，Changchun 130022，Jilin，China |
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