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投稿时间：2019-04-19
投稿时间：2019-04-19
中文摘要: 以蓝莓叶为原料，采用超声波辅助提取黄酮类化合物，用正交试验对提取工艺进行优化，并考察提取物总黄酮对毛尖茶叶脂肪氧合酶（lipoxygenase）的抑制作用。结果表明：超声波辅助提取总黄酮的优化条件为乙醇体积分数60%，料液比1∶50（g/mL），提取温度70 ℃，提取时间30 min，优化条件下蓝莓叶总黄酮提取率为9.56%。蓝莓叶中黄酮类化合物对毛尖茶叶脂氧合酶有一定的抑制作用，IC50 为22.3 μg/mL。
Abstract:Using blueberry leaves as raw material，the flavonoids was extracted by ultrasonic-assisted method，and the extraction process was optimized by orthogonal test，and the inhibitory effect of total flavonoids on the lipoxygenase of Maojian tea was studied.The results showed that the optimal conditions for ultrasonic-assisted extraction of total flavonoids were：the volume fraction of ethanol was 60 %，the ratio of material to liquid was 1∶50（g/mL），the extraction temperature was 70 ℃，and the extraction time was 30 min，under the optimized conditions，the extraction yield of total flavonoids was 9.56 %.Flavonoids in blueberry leaves had a certain inhibitory effect on lipoxygenase in Maojian tea，the IC50 was 22.3 μg/mL.
keywords: blueberry leaves flavonoids optimization Maojian tea leaves lipoxygenase inhibitory effects
文章编号：202008009 中图分类号： 文献标志码：
基金项目:2017年度贵州省教育厅青年科技人才成长项目（黔教合KY字[2017]349）；2017年黔南民族师范学院校级重点项目（qnsy2017002）
| Author Name | Affiliation |
| WU Gui-ling，HU Li，FENG Ding-kun，XING Yan | School of Chemistry and Chemical Engineering，Qiannan Normal University for Nationalities，Duyun 558000，Guizhou，China |
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