Abstract:The fish mince wash water was used as raw material to study.The structure and functional properties of the silver carp sarcoplasmic protein recovered by different recovery methods such as 35 ℃vacuum concentration and lyophilization（LT），35 ℃ vacuum concentration-freeze-drying by adjusting pH（LT-pH），65 ℃ vacuum concentration freeze drying（HT），65 ℃ vacuum concentration-pH-shifting freeze-drying（HT-pH）and pH-shifting freeze-drying （pH）were study.Fourier transform infrared spectroscopy revealed the αhelix and β-sheet of the sarcoplasmic protein recovered by five methods were lost compared with the crude，and the structure and conformation change of the sarcoplasmic protein recovered by pH-shifting were larger than the other groups；scanning electron micrograph indicated that all of the five recovery methods could destroy sarcoplasmic protein with different degrees.The functional characteristics indicated that the total sulfhydryl content of the LT group had the largest change compared with the other group，the surface hydrophobicity，the emulsifying activity index and emulsification stability of pH-shifting had the largest change compared with the other group，the denaturation temperature of the HT-pH had the largest change compared with the other group.The results showed that the structure and functional characteristics of silver carp sarcoplasmic protein varied with different recovery methods.