Abstract:A headspace solid phase microextrction （HS-SPME） coupled to gas chromatography mass spectrometry（GC-MS）method was developed for the determination of volatile compound of lychee honeys from the different regions in China.Under the optimized conditions，ninety-eight different volatile compounds were identified，mainly including terpenes，alcohols，aldehydes，and lactones，with linalool oxide as the dominate compound.After compared honey volatiles from different honeybee species，the results suggested that possible markers for honeys produced by Apis cerana cerana were 1-heptanol，1-nonanol，lilac alcohol，phenylethyl acetate，while decanal，epoxy-linalooloxide and unknown chromatographic peak （RT：27.98） for honeys produced by Apis mellifera ligustica.The analyzed samples of lychee honeys（both Apis mellifera ligustica and Apis cerana cerana）had presented a distinctive profile by those of different volatile compounds.