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投稿时间:2019-04-23
投稿时间:2019-04-23
中文摘要: 以长白山人参茎叶为原料,采用水提醇沉淀法提取人参茎叶中的多糖,用苯酚-硫酸法测定多糖的含量。以多糖提取率为指标,通过单因素试验和正交试验设计对提取工艺进行优化。评价提取温度、提取时间、料液比、pH 值4 个因素对多糖提取效果的影响,并确定最优提取工艺为:提取温度90 ℃,提取时间4 h,料液比1 ∶25(g/mL)、pH 5.0。在此条件下,人参茎叶多糖的提取率为5.64%。
Abstract:Polysaccharide was extracted from Changbai mountain ginseng stems and leaves by water extraction and alcohol precipitation.The content of polysaccharide was determined by phenol-sulfuric acid method,and the extraction rate was as an index.The extraction technology was optimized by the single factor experiment and the orthogonal test design.The extraction temperature,extraction time,material-to-water ratio and pH of 4 factors on extraction rate of polysaccharide were evaluated.Ultimately the optimal extraction condition was as follows:extraction temperature 90 ℃,extraction time 4 h,material-to-water ratio 1 ∶25(g/mL),pH 5.0.In thess conditions,the extraction rate of polysaccharide in ginseng stems and leaves was 5.64%.
文章编号:202006017 中图分类号: 文献标志码:
基金项目:
Author Name | Affiliation |
SUN Xiao-ling | Food and Biology Department of Changchun Vocational Institute of Technology,Changchun 130033,Jilin,China |
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