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投稿时间：2019-04-05
投稿时间：2019-04-05
中文摘要: 为提高屎肠球菌B13 乳酸菌素的产量，以接种量、培养基初始pH 值和发酵温度为考察因素，在单因素试验的基础上，利用Box-Behnken 中心组和试验设计原理进行三因素三水平的响应面优化分析，优化后的最佳培养条件为：接种量4.4%、培养基初始pH 5.3、发酵温度33 ℃。在此条件下，乳酸菌素的抑菌效价达5.32×108 IU/mL，比优化前提高1.44 倍，为屎肠球菌B13 乳酸菌素的开发利用提供参考依据。
Abstract:In order to improve the bacteriocin production of Enterococcus faecium B13，inoculum，initial pH of the medium and fermentation temperature was taken as an investigation factor，on the basis of single factor experiment，four factors were designed using Design Expert 8.0.6 Box-Behnken center composite design .The optimal fermentation conditions were inoculum amount of 4.4%，initial pH of 5.3，fermentation temperature of 33 ℃.Under this condition，the bacteriocin inhibitory activity was 5.32×108 IU/mL，which was 1.44 times higher than before，it provides a reference for the development and utilization of Enterococcus faecium B13 lactobacillus.
keywords: Enterococcus faecium B13 response surface methodology bacteriocin produced by lactic acid bacteria inhibitory activity optimization of fermentation conditions
文章编号：202005032 中图分类号： 文献标志码：
基金项目:西南民族大学研究生“创新型科研项目”（CX2017SZ045）
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