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食品研究与开发:2020,41(5):164-170
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内蒙古乌海地区沙漠葡萄发酵醪液中酿酒酵母菌的筛选与鉴定
(1.内蒙古农业大学职业技术学院,内蒙古包头014109;2.内蒙古农业大学食品科学与工程学院,内蒙古呼和浩特010018)
Screening and Identification of Saccharomyces cerevisiae in Fermentation Mash of Desert Grape in Wuhai Area of Inner Mongolia
(1.Vocational and Technical College,Inner Mongolia Agricultural University,Baotou 014109,Inner Mongolia,China;2.College of Food Science and Engineering,Inner Mongolia Agricultural University,Hohhot 010018,Inner Mongolia,China)
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投稿时间:2019-10-26    
中文摘要: 以内蒙古乌海地区沙漠葡萄自然发酵醪液为筛选来源,共分离纯化出22 株酵母菌,通过复筛得到1 株优良酵母菌KT3,经26S rDNA D1/D2 区测序,鉴定为酵母菌属(Saccharomyces)的酿酒酵母菌(Saccharomyces cerevisiae);该酵母菌具有良好的耐受能力,耐酒精度为16%,耐SO2 浓度为450 mg/L,耐高糖浓度为50%,耐pH 值为2.5;并且具备高产酒精和低产硫化氢的能力,发酵产品风味协调,典型性强,可以用于葡萄酒的生产。
中文关键词: 酿酒酵母  葡萄酒  发酵醪液  筛选  鉴定
Abstract:22 strains of yeast in Wuhai area of Inner Mongolia were isolated and purified from natural fermentation mash.One strain of yeast KT3 was obtained through re-screening.The yeast was identified as Saccharomyces cerevisiae by sequencing in 26S rDNA D1/D2 region.The yeast had good tolerance.Alcohol tolerance was 16 %,sulfur dioxide resistant concentration was 450 mg/L,sugar tolerance was 50 %,pH tolerance was 2.5.Furthermore,it had the ability of high-yield alcohol and low-yield hydrogen sulfide.The fermented product could be used in wine production since it had harmonious flavor and strong typicality.
文章编号:202005027     中图分类号:    文献标志码:
基金项目:内蒙古自治区教育厅产学研科技创新平台项目(NJCXY-19-05)
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