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投稿时间：2019-12-01
投稿时间：2019-12-01
中文摘要: 分别以水、70%甲醇、70%乙醇和70%丙酮为提取剂，超声辅助提取金银花中多酚，采用Folin-Ciocalteu 法测定提取液中多酚量，并利用清除DPPH·、·OH 和ABTS＋·法评价其抗氧化活性。结果表明，金银花不同溶剂水、70%甲醇、70%乙醇和70%丙酮提取物中多酚含量分别为18.97、46.56、46.63、48.49 mg/g，以水为溶剂与以有机溶液为溶剂，金银花多酚提取量有极显著差异（p＜0.01）。不同溶剂金银花多酚均具有一定的清除DPPH·、·OH 和ABTS＋·能力，即抗氧化活性，但其清除 DPPH·和 ABTS＋·的能力弱于 2，6-二叔丁基-4-甲基苯酚（butylated hydroxytoluene，BHT），清除·OH 的能力强于BHT。不同溶剂提取的金银花多酚种类不同，对自由基的清除能力不同。
Abstract:Honeysuckle polyphenols were extracted by ultrasonic-assisted with water，70 % methanol，70 %ethanol，and 70 % acetone as extractant，respectively.The content of polyphenols was determined by Folin-Ciocalteu method，and the antioxidant activities were evaluated by scavenging DPPH·，·OH and ABTS＋·.The results showed that the content of polyphenols in water，70 % methanol，70 % ethanol and 70 % acetone extractions were 18.97，46.56，46.63 mg/g and 48.49 mg/g，respectively.The amounts of honeysuckle polyphenols were significantly different between the extraction with water as extractant and the extraction with organic solvent as extractant（p<0.01）.Honeysuckle polyphenols from different solvent all had certain ability to scavenge DPPH·，·OH and ABTS＋·，namely antioxidant activity.However，the ability to scavenge DPPH·and ABTS＋·was weaker than butylated hydroxytoluene （BHT），and the ability to scavenge·OH was stronger than BHT.The types of honeysuckle polyphenols extracted by different solvents were different，and the abilities of scavenging free radicals were different.
文章编号：202005017 中图分类号： 文献标志码：
基金项目:国家级大学生创新创业训练计划项目（201910124008）；山西省1331 工程重点学科建设计划支持项目（2019）；山西省高等学校大学生创新创业训练计划项目（2019514）；忻州师院化学化工大学生创新基地项目（2019）
| Author Name | Affiliation |
| WANG Gui-lin，XU Wei-fang，LIU Le，FAN Jian-feng，ZHAO San-hu，ZHAO Er-lao | Xinzhou Teachers University，Xinzhou 034000，Shanxi，China |
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