Abstract:Using Wutai Mountain Clitocybe squamulosa （Pers.） Kumm as the raw material，the response surface analysis method was used to optimize the extraction process of Clitocybe squamulosa protein which was analyzed for amino acid composition and nutritional value.Clitocybe squamulosa protein isolated was based on the results of single factor experiments，and a central composite design including independent variables such as the liquid-to-material ratio，the pH value of extraction and the extraction temperature were conducted by the response surface methodology.The optimal extraction conditions were as follows：liquid-to-material ratio of 31 mL/g，the extraction pH of 11，extraction temperature of 51 ℃，and extraction time of 60 min，the protein extraction rate reached （33.65±0.17）%.Under these conditions，the nutritional value analysis of Clitocybe squamulosa protein showed that the essential amino acids（EAA）accounted for 39.35%of the total amino acids（TAA），and the ratio of essential amino acids to non-essential amino acids （NEAA）was 64.89%，the sample protein is close to the recommended value proposed by the Food and Agriculture Organization （FAO）/World Health Organization（WHO）.Therefore，the Clitocybe squamulosa protein isolated is a high-quality protein.