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投稿时间：2019-03-26
投稿时间：2019-03-26
中文摘要: 为建立氯唑西林（cloxacillin，CLOX）残留的酶联免疫（enzyme-linked immuno sorbent assay，ELISA）检测方法，试验采用碳化二亚胺法，将氯唑西林偶联于载体蛋白牛清蛋白（Bovine albumin，BSA）和卵清蛋白（ovalbumin，OVA），制得免疫抗原（CLOX-BSA）和包被抗原（CLOX-OVA），并通过红外光谱扫描（infrared spectroscopy，IR）鉴定表明CLOX 人工抗原合成成功。用免疫抗原免疫5 只小鼠，制备CLOX 多克隆抗体，通过间接ELISA 法测定其对CLOX 的效价及特异性。结果表明：用CLOX-BSA 免疫制备的抗体效价达1 ∶64 000 以上，特异性较强，对CLOX 的半抑制质量浓度 IC50 值为（5.64±0.03）ng/mL，IC15 值为（22.43±0.02）ng/mL。说明试验得到了灵敏度高、特异性强及具有开发性的抗体，可为食品安全检验试剂盒的开发提供基础。
Abstract:A method for detecting residual enzyme-linked immunosorbent（ELISA）of cloxacillin（CLOX）was established.In the experiment，CLOX-BSA and CLOX-OVA were prepared by coupling cloxacillin to carrier protein bovine albumin （BSA）and ovalbumin （OVA）by carbodiimide method，and the synthesis of CLOXBSA and CLOX-OVA was proved to be successful by IR scanning.KM mice were immunized with immunizing antigen to prepare CLOX polyclonal antibody，and its titer and specificity for CLOX were determined by indirect ELISA.The results showed that the preparation with CLOX-BSA immune antibody titer for 1 ∶64 000 above，strong specificity，quality of CLOX half inhibition concentration of （5.64±0.03）ng/mL，and the IC15 value was（22.43±0.02）ng/mL.It showed that the experiments had obtained high sensitivity，specificity and developmental antibodies，which could provide a basis for the development of food safety testing kits.
文章编号：202004018 中图分类号： 文献标志码：
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