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食品研究与开发:2020,41(4):38-44
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响应面优化紫果西番莲多糖提取工艺及抗氧化活性研究
(1.黔南民族师范学院化学化工学院,贵州都匀558000;2.贵州省高校民族药用植物资源开发工程研究中心,贵州都匀558000)
Response Surface Methodology for Optimization of Extraction and Antioxidant Activity of Polysaccharides in Purple Passionfruit
(1.School of Chemistry and Chemical Engineering,Qiannan Normal University for Nationalities,Duyun 558000,Guizhou,China;2.Guizhou Province College Research Center of Ethnical Medicinal Plant Resources Ex-ploitation Engineering,Duyun 558000,Guizhou,China)
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投稿时间:2019-04-01    
中文摘要: 以紫果西番莲为研究对象,采用单因素试验和响应面分析法优化紫果西番莲果肉多糖的提取工艺,考察液料比、超声时间、超声功率和超声温度对其多糖提取量的影响;以清除DPPH 自由基和·OH 能力评价紫果西番莲果肉多糖的抗氧化活性。结果表明:紫果西番莲果肉多糖最佳提取工艺为:液料比5 mL/g、超声时间20 min、超声功率330 W和超声温度70 ℃,测得紫果西番莲多糖的提取量为98.82 mg/g;紫果西番莲果肉多糖有一定的DPPH 自由基和·OH的清除能力,其清除 DPPH 自由基、·OH 的半抑制浓度(IC50)分别为 66.97 μg/mL 和 0.23 mg/mL。
中文关键词: 紫果西番莲  响应面分析  多糖  提取  抗氧化
Abstract:Purple passionfruit as the study object,effects of solvent to material ratio,ultrasonic time and ultrasonic power on the extraction of polysaccharides were studied by single factor and response surface methodology in the purple passionfruit.The optimal extraction technology was as follow:solvent to material ratio 5 mL/g,ultrasonic time 20 min,ultrasonic power 330 W and ultrasonic temperature 70 ℃.Under the above conditions,the polysaccharides of purple passionfruit was 98.92 mg/g.The activity test showed that the polysaccharides of purple passionfruit could scavenge DPPH and hydroxyl radicals,and the half inhibitory concentration (IC50)for scavenging of DPPH and hydroxyl radicals were 66.97 μg/mL and 0.23 mg/mL,respectively.
文章编号:202004007     中图分类号:    文献标志码:
基金项目:贵州省科学技术联合基金项目(LH 字[2015]7714);贵州省教育厅青年科技人才成长项目(黔教合KY 字[2018]431);贵州省教育厅自然科研青年项目(黔教科[2014]314 号);校级产学研基地(Qnsyk201603)
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