Abstract:The extract of passion fruit seed has a variety of biological activities and the active substance may come from metabolic product of bacteria.This article discussed the separation and identification of endogenous bacteria in Liuzhou passion fruit seeds.Using the fresh passion fruit，the endogenous bacteria of the fruit seeds were separated and purified by plate count agar （PCA）culture medium，and then identified it by molecular biology.Finally，the biological characteristics of the optimum growth temperature，the optimal pH value and the growth curve were measured.The results showed that the colonies isolated from the passion fruit seeds were white.All of these bacteria were gram-positive coccus，the diameter of the coccus between was 1.3 μm-1.9 μm.Random selection of 3 strains of bacteria for molecular biology was belonging to the Acinetobacter.Besides，the bacteria grow in the LB culture medium were fine，and its optimal pH value were 7.0.The optimum growth temperature of these bacteria were 25 ℃ and the growth inhibition temperature were ＜15 ℃ or ＞ 40 ℃.The logarithmic period of the strains was from 6h to 16h after inoculation.This paper found that Acinetobacter exist－ed in the Liuzhou passion fruit seeds by separation and identification of endogenous bacteria in the seeds of the passion fruit.