Abstract:The antibacterial films were prepared by using starch as film matrix，sodium alginate and glycerin as plasticizers，and epsilon-poly lysine as the antibacterial agents.And its antibacterial and properties were tested.Results showed that the minimal inhibitory concentrations （MIC）of epsilon-poly lysine to Escherichia coli，bacillus subtilis and yeast was 1，2，2 μg/mL，respectively.When epsilon-poly lysine was added to the starch film，their MIC were increased to 64 mg/g.The mechanical properties，infrared spectroscopy，permeability，gas permeability and oil permeability of antimicrobial film were tested and compared to starch film.Their film fluids were also compared with rheological property and contact angle.All properties of the both films are not significant differences.