Abstract:The crude polysaccharides were extracted from the sophorae fructus by ultrasonic -complex enzymatic hydrolysis with water extraction and alcohol precipitation method.Sophorae fructus polysaccharides（SFP）were purified by Sevage reagent.The secondary polysaccharides SFP-1 and SFP-2 were separated and purified by DEAE-52 cellulose column，with sophorae fructus polysaccharides （SFP），SFP-1 and SFP-2 as experimental materials，the antioxidant effects of ABTS+free radical，DPPH free radical，superoxide anion free radical and hydroxyl radical were studied by free radical evaluation method，and the inhibitory effects of polysaccharides on DNA damage were investigated.The results showed that the antioxidant activity of sophorae fructus polysaccharides and its purified components （SFP-1，SFP-2）to four free radicals were dose-effect relationship.At the concentration of 6.4 mg/mL，ABTS+free radical scavenging rate was higher than 99.73%，DPPH free radical scavenging rate was up to 87.05%，superoxide anion free radical scavenging rate was up to 50.92 %，hydroxyl radical scavenging rate was up to 96.54 %，and inhibited DNA chain opening and ring unwrapping.Polysaccharides from sophorae fructus had good antioxidant activity and have certain development value.