Abstract:The purpose of this experiment was to provide further data reference and process optimization for the extraction of lycopene.In this experiment，passion fruit（Passiflora edulia Sims）was used as the raw material of lycopene extraction，single factor test and orthogonal test were set up to compare and analyze the two experiments，so as to study the best extraction technology.Five factors were set up in single factor experiment：pretreatment method，extraction agent，extraction time，extraction temperature，liquid-to-material ratio.Based on the results of single factor test，three factors （extraction time，extraction temperature，liquid-to-material ratio）were selected to design orthogonal test.Through the comparison and analysis of single factor test and orthogonal test，the best pretreatment method was centrifugation in 15 000 r/min high speed centrifuge for 15 min，the optimum extraction technology was among the factors affecting the amount of lycopene extraction，the ratio of liquid to material was the main one，the extraction time and temperature.acetone-n-hexane 2 ∶1（volume ratio）as extracting agent，liquid-to-material ratio of 3 ∶1（mL/g）.The extract was extracted for 3 h in a constant temperature oscillation incubator at 35 ℃.