Abstract:In order to explore the effects of different soaking time on the organic acid components in the Lonicera japonica Flos aqueous extract，the Lonicera japonica Flos was soaked at 0，30，60，90 min and extracted at 80，95 ℃.The content of the seven active ingredients was determined by high performance liquid chromatography（HPLC）.The extraction effect of chlorogenic acid was the best after soaking for 30 min.The extraction effect of sweroside was the best after soaking for 90 min.The extraction effects of cryptochlorogenic acid，isochlorogenic acid A and isochlorogenic acid C were as follows：direct extraction > soak for 30 min >soak for 90 min and soak for 60 min.The extraction effect of chlorogenic acid，neochlorogenic acid，cryptochlorogenic acid，isochlorogenic acid B and isochlorogenic acid C at 95 ℃ was better than that at 80 ℃.Isochlorogenic acid A was better at 80 ℃ than at 95 ℃.The results showed that soaking had different effects on various organic acid components in Lonicera japonica Flos.The extraction conditions of 90 min extraction at 95 ℃after soaking for 30 min could ensure the higher organic acid extraction amount in the Lonicera japonica Flos，and ensure the production efficiency.Appropriate soaking could speed up the extraction process，increase the yield，and reduce the energy consumption of heating extraction for a long time.