Abstract:The effect of Curcuma longa L.volatile oil on apoptosis of human lung cancer A549 cells and its mechanism was studied.Adopted by methyl thiazole blue colorimetry （MTT）to detect the inhibitory effect of Curcuma longa L.volatile oil on cell proliferation of A549.The mechanism of apoptosis induced by Curcuma longa L.volatile oil was investigated by acridine orange（AO）staining，flow cytometry，scratch and colony formation experiments.The results showed that Curcuma longa L.volatile oil inhibitd the proliferation of cancer cells in a time-and dose-dependent manner.When the concentration of Curcuma longa L.volatile oil was 100 mg/L，the cell structure could be obviously deformed and the nucleus could shrink and apoptosis.At the same time，compared with the blank control group，the number of apoptotic cells in the early and late stages of A549 cells treated with different concentrations of Curcuma longa L.volatile oil increased，and their migration ability was also inhibited to varying degrees.