Abstract:The present study aimed to screen the optimal extraction solvent and shell parts，and to investigate the anti-hypertensive effect of sacha inchi shell via ACE inhibition in vitro in combination with the spontaneously hypertensive rat （SHR）in vivo.The results of in vitro tests showed that sacha inchi shell water extract（SISE）exhibited the highest angiotensin-converting enzyme （ACE）inhibition rate，which was significantly higher than other natural ACE inhibitors（chrysanthemum extract and ginkgo biloba extract）.The results of in vivo experiments indicated that the blood pressure of SHR were significantly decreased at the high-dose group（400 mg/kg·BW），which decrease the systolic blood pressure of SHR from 166 mmHg to 135 mmHg.The results first confirmed the anti-hypertensive effect of SISE.Acute oral toxicity test showed that SISE was a foodgrade non-toxic.The active ingredients were further separated and the effective anti-hypertensive components were mainly concentrated in n-butanol extracts.This extract was eluted through a silica gel column with a gradient of chloroform/methanol，and the chloroform/methanol（7 ∶3）components had the highest inhibition effect of ACE.The present result will provide help in developing sacha inchi shell as novel antihypertensive drugs.