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食品研究与开发:2019,40(22):1-6
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新疆黑蜂蜂王浆球蛋白酶解产物的抗氧化活性
JIANG Jian-hui,ZHOU Xin,CUI Tian-yi,CHEN Long
Antioxidant Activity of the Enzymatic Products of Globulin from the Royal Jelly of Xinjiang Black Bees
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投稿时间:2019-01-11    
中文摘要: 为评价新疆黑蜂蜂王浆球蛋白酶解产物的抗氧化活性,以胰蛋白酶为试验用酶,采用清除二苯基-2-苦肼自由基(DPPH·),抑制亚油酸氧化和总抗氧化能力测定方法,探讨新疆黑蜂球蛋白酶解产物的水解条件。结果表明当温度为57 ℃、pH 值为7.5、酶底比为1.5%(质量分数)、酶解时间为4 h 时对DPPH·清除率最大,抗氧化活性最强。该结论对研制具有不同抗氧化功能的新疆黑蜂蜂王浆制品,具有一定的理论指导价值。
中文关键词: 蜂王浆  抗氧化  酶解  酶解产物  球蛋白
Abstract:In order to evaluate the antioxidant activity of the proteolytic products of the royal jelly of Xinjiang black bee,the antioxidative effect of trypsinized globin from the royal jelly protein was investigated.This study used trypsin as the experimental enzyme,and the antioxidation effect of the proteolytic products was determined by measuring the removal of diphenyl-2-bitrinium free radical (DPPH·),the inhibition of linoleic acid oxidation and the total antioxidation ability.The optimum hydrolysis conditions for the proteolytic products of the globin from the royal jelly of Xinjiang black bee was determined as 57 ℃,pH=7.5 and E/S ratio 1.5 %(mass fraction)for 4 h,at which the products showed the highest DPPH·scavenging rate and antioxidant activity.This research had certain theoretical guiding value for the development of Xinjiang black bee royal jelly products with antioxidant functions.
文章编号:201922001     中图分类号:    文献标志码:
基金项目:国家自然科学基金(21562035)
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