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食品研究与开发:2019,40(21):209-213
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传统东北酸菜中乳杆菌的分离鉴定及其降胆固醇特性研究
MA Chang-lu,JIAO Meng-li,LUO Hong-xia,PANG Xiao-yang,LU Jing,ZHANG Shu-wen,Lü Jia-ping,TUO Yan-feng
(1.Department of Food and Bio-engineering,Beijing Vocational College of Agriculture,Beijing 102442,China;2.Institute of Food Science and Technology,Chinese Academy of Agricultural Sciences,Beijing 100193,China;3.College of Food Science and Engineering,Beijing University of Agricultural,Beijing 102206,China;4.School of Food Science and Technology,Dalian Polytechnic University,Dalian 116034,Liaoning,China)
Isolation and Identification of Lactic Acid Bacteria from Traditional Northeast Pickled Vegetables and Their Cholesterol-Lowering Properties
1.Department of Food and Bio-engineering,Beijing Vocational College of Agriculture,Beijing 102442,China;2.Institute of Food Science and Technology,Chinese Academy of Agricultural Sciences,Beijing 100193,China;3.College of Food Science and Engineering,Beijing University of Agricultural,Beijing 102206,China;4.School of Food Science and Technology,Dalian Polytechnic University,Dalian 116034,Liaoning,China
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投稿时间:2019-01-02    
中文摘要: 以自然发酵的传统东北酸菜为样品来源,分离得到10 株革兰氏阳性、过氧化氢酶阴性、有明显透明溶钙圈的乳酸菌,通过分子和生化手段对其生物学分类地位进行鉴定为乳杆菌属微生物。通过邻苯二甲醛比色法测定其从培养基中脱除胆固醇的能力,发现分离获得的植物乳杆菌MHS1701、干酪乳杆菌MHS1703、棒状乳杆菌MHS1706,从培养基中脱除胆固醇的脱除率分别为40.71%、52.84%、41.41%,植物乳杆菌MHS1701 的体外低pH 值、高胆盐耐受性优于其他两株菌。
中文关键词: 东北酸菜  乳酸菌  降胆固醇  耐受性  筛选  鉴定
Abstract:Ten strains of Gram-positive and catalase negative lactic acid bacteria were isolated from the natural fermented traditional northeast pickled vegetables.Its biological taxonomic status was identified as Lactobacillus by molecular and biochemical methods.The ability of removing cholesterol from culture medium was determined by phthalaldehyde colorimetry.It was found that the removal rate of cholesterol from culture medium by Lactobacillus plantarum MHS1701,Lactobacillus casei MHS1703,Corynebacterium MHS1706,was 40.71%,52.84 %,41.41 % spectively.The tolerance of low pH value,and high bile salt tolerance of Lactobacillus MHS1701 in vitro was superior to that of the other two strains.
文章编号:201921034     中图分类号:    文献标志码:
基金项目:国家自然科学基金面上项目(31871833、31371808);北京市教委科技计划项目(KM201812448003);国家重点研发计划专项(2017YFC1600903)
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