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投稿时间:2019-01-02
投稿时间:2019-01-02
中文摘要: 以自然发酵的传统东北酸菜为样品来源,分离得到10 株革兰氏阳性、过氧化氢酶阴性、有明显透明溶钙圈的乳酸菌,通过分子和生化手段对其生物学分类地位进行鉴定为乳杆菌属微生物。通过邻苯二甲醛比色法测定其从培养基中脱除胆固醇的能力,发现分离获得的植物乳杆菌MHS1701、干酪乳杆菌MHS1703、棒状乳杆菌MHS1706,从培养基中脱除胆固醇的脱除率分别为40.71%、52.84%、41.41%,植物乳杆菌MHS1701 的体外低pH 值、高胆盐耐受性优于其他两株菌。
Abstract:Ten strains of Gram-positive and catalase negative lactic acid bacteria were isolated from the natural fermented traditional northeast pickled vegetables.Its biological taxonomic status was identified as Lactobacillus by molecular and biochemical methods.The ability of removing cholesterol from culture medium was determined by phthalaldehyde colorimetry.It was found that the removal rate of cholesterol from culture medium by Lactobacillus plantarum MHS1701,Lactobacillus casei MHS1703,Corynebacterium MHS1706,was 40.71%,52.84 %,41.41 % spectively.The tolerance of low pH value,and high bile salt tolerance of Lactobacillus MHS1701 in vitro was superior to that of the other two strains.
keywords: northeast sauerkraut lactic acid bacteria cholesterol lowering tolerance screening identification
文章编号:201921034 中图分类号: 文献标志码:
基金项目:国家自然科学基金面上项目(31871833、31371808);北京市教委科技计划项目(KM201812448003);国家重点研发计划专项(2017YFC1600903)
作者 | 单位 |
马长路,焦梦丽,罗红霞,逄晓阳,芦晶,张书文,吕加平,妥彦峰 | 1.北京农业职业学院食品与生物工程系,北京102442;2.中国农业科学院农产品加工研究所,北京100193;3.北京农学院食品科学与工程学院,北京102206;4.大连工业大学食品学院,辽宁大连116034 |
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