Abstract:For the further development and utilization of honeysuckle resources，the response surface methodology was used to optimize extraction process of chlorogenic acid from Lonicera japonica.The oxidation resistance of chlorogenic acid was analysed.The result showed that the concentration of ethanol was 69%，ratio of solid-liquid was 1 ∶23（g/mL），ultrasonic temperature was 44 ℃，ultrasonic time was 20 min.Under the optimization condition，the extraction rate of honeysuckle chlorogenic acid was 6.20 %.The IC50 of DPPH free radical of chlorogenic acid from Lonicera japonica was 21.09 μg/mL，so chlorogenic acid had stronger antioxidant activity.The synergistic effect between chlorogenic acid and rutin was analysed.When the mix proportion of honeysuckle chlorogenic acid and rutin was 9 ∶1，the coordination coefficient of SE was 1.09.The synergies effectbetweenthem was obvious.