Abstract:In order to improve the extraction yield of sesame oil，the ultrasound and freeze-microwave thawing assisted aqueous enzymatic extraction was used to extract oil from sesame，single factor experiments and orthogonal test had been applied to optimize the conditions of ultrasonic pretreatment and freeze-microwave thawing pretreatment.The results showed that the ultrasonic pretreatment conditions were as follows：ratio of material to liquid 1 ∶6（g/mL），ultrasonic time 30 min，ultrasonic power 60%（total power 500 W），ultrasonic temperature 50 ℃，unde the above conditions，the sesame oil yield was 81.32 %；the freeze-microwave thawing pretreatment conditions were as follows：crushing time 24 s，freezing time 20 h，freezing temperature-20 ℃，the microwave thaw time 12 min，under the above conditions，the sesame oil yield was 85.23 %.According to comparing the quality index properties of sesame oil，the results showed that the quality of the sesame oil obtained by ultrasound pretreatment was better than the sesame oil by freeze-microwave thawing pretreatment，and two enzyme-assisted aqueous extraction processing（EAEP）of sesame oil meet the standard of refined sesame oil.