Abstract:The purification process of total flavonoids from wild artemisia by macroporous adsorption resin and its antioxidant activity in vitro were investigated.The adsorption and desorption capacity of seven kinds of macroporous resins to total flavonoids of the artemisia were studied with its total flavonoid adsorption and desorption as response values.The optimum resin type was AB-8，and the antioxidative activities of total flavone to ABTS+ was explored by dynamic adsorption and desorption experiment.The results showed that a better process for purification of total flavonoids from Artemisia by AB-8 resin was as follows：the concentration of flavonoids extraction solution was 1 mg/mL，pH 4，the sample loading and flow rate of the sample were 5 BV and 2 BV/h，the eluent was 60%volume fraction of ethanol solution with a dosage of 4 BV.Under the optimum conditions，the total flavonoids content in the purified extract was increased from 13.6%to 52.4%.The results of antioxidant activity in vitro showed that the total flavonoids of artemisia had a certain antioxidant activity to ABTS+，which increased with the increase of mass concentration.