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投稿时间:2019-08-07
投稿时间:2019-08-07
中文摘要: 为研究意大利SACCO WBL-45 复合菌种和意大利SACCO VBL-97 复合菌种在天然腌制肉制品中的应用效果,先利用复合菌种的硝酸盐还原作用发酵天然芹菜汁,以期得到富含亚硝酸盐的发酵芹菜冻干粉,再分别应用于制作天然腌制灌肠并研究效果。分别设定 VBL-97 菌株接种量为 6.25×105、1.25×106、6.25×106、2.5×107、6.25×107 CFU/g,WBL-45 菌株接种量为 7.08×105、1.42×106、7.08×106、2.83×107、7.08×107 CFU/g,发酵温度为 38 ℃,在 0、12、24、60、96、120、150 h 测定发酵芹菜液pH 值、硝酸盐含量、亚硝酸盐含量,研究芹菜液发酵规律,以亚硝酸盐生成量初步选定复合菌种接种量和发酵时间,其次将发酵完成的芹菜汁冻干为WBL-45 复合菌种发酵芹菜粉和VBL-97 复合菌种发酵芹菜粉,分别制作天然腌制灌肠,以化学合成型亚硝酸钠制作灌肠作为对照组,在0、1、3、5、7d 测定a*值、亚硝酸盐残留和硫代巴比妥酸值(thiobarbituric acid reactive substances,TBARS),研究其腌制效果。试验结果表明,WBL-45 接种量为1.42×106 CFU/g 时,VBL-97 接种量为1.25×106 CFU/g 时,发酵进行到24 h 左右,发酵芹菜液pH 值和硝酸盐含量均降到最低,亚硝酸盐生成量达到峰值,分别为1 013 mg/kg 和256 mg/kg,WBL-45 组硝酸盐转化量更高(P<0.05),两种发酵芹菜粉制作灌肠在a*上与对照组存在差异但相差不大,在TBARS 值和亚硝酸盐残留方面优于对照组,同时WBL-45 发酵芹菜粉灌肠的a*值要比VBL-97 组高。
Abstract:To research the effect of green cured sausages produced by Italy SACCO WBL-45 compound microorganisms and VBL-97 compound microorganisms,fermented celery powder rich in nitrite would be obtained by nitrate reduction from compound microorganisms fermenting celery juice,which made green cured sausages and studied the curing effect.The inoculation concentration of WBL-45 compound microorganisms was 7.08×105,1.42×106,7.08×106,2.83×107,7.08×107 CFU/g,while the inoculation concentration of VBL-97 was 6.25×105,1.25×106,6.25×106,2.5×107,6.25×107 CFU/g,the nitrite concentration,pH and nitrate concentration were measured at 0,12,24,60,96,120,150 h along with 38 ℃ fermentation temperature.The inoculation concentration and fermentation time would be selected by the nitrite concentration in celery juice.The quality of green cured sausages produced by two celery powder dated from above-mentioned best celery juice was studied by measuring residual nitrite concentration,thiobarbituric acid reactive substances(TBARS)and a*at 0,1,3,5,7 d,while chemosynthetic nitrite cured sausages as control.Results reflected that the nitrite concentration increased to maximum and the pH,nitrate concentration both decreased to minimum at 24 h with WBL-45 1.42×106 CFU/g and VBL-97 1.25×106 CFU/g,when WBL-45 group residual nitrite concentration 1 013 mg/kg more than VBL-97 group residual nitrite concentration 256 mg/kg(P<0.05).The a*of WBL-45 fermentation celery powder sausage was higher than VBL-97,but both had no significant difference for control group.Meanwhile,the thiobarbituric acid reactive substances(TBARS)and residual nitrite concentration were more excellent than control.
keywords: celery compound microorganisms nitrite sausage creen cured
文章编号:201920009 中图分类号: 文献标志码:
基金项目:“十三五”国家重点研发计划重点专项(2016YFD0401500);山西省自然科学基金项目“发酵蔬菜粉抑制肉毒梭状芽孢杆菌及N-亚硝胺形成的机理研究”(201701D121104)
作者 | 单位 |
李沛然,朱迎春,杨华,梁丽雅,马俪珍 | 天津农学院总务处,天津300384;山西农业大学食品科学与工程学院,山西晋中030801;天津农学院动物科学与动物医学学院,天津300384;天津农学院食品科学与生物工程学院,天津300384 |
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