Abstract:To develop and apply a method for the simultaneous determination of 13 kinds of illegal drugs in antidiabetic supplements by using high performance liquid chromatography-tandem mass spectrometry（HPLCMS/MS）.The samples were extracted using methanol.The target analytes were separated on column（2.1 mm×150 mm，5 μm）with a mobile phase consisting of 10 mmol/L ammonium acetate（including 0.1%formic acid）and methanol by gradient elution.The detection was determined based on ESI+（electrospray ionization positive）and MRM （multiple reaction monitoring） mode.The results showed good linear relationships with the correlation coefficients larger than 0.999 for all 13 kinds of illegal drugs.The limits of quantitation （LOQ）were all in the range of 45.7 μg/kg-79.6 μg/kg.The recoveries were in the range of 78.6 %-112.1 % with relative standard deviations （RSD）of 0.6 %-3.5 % at three spiked levels.In the 11 batches of samples，the illegal synthetic drugs were found in 5 samples and all the 5 samples contain more than one illegal synthetic drug；in which metformin was found in two samples，phenformin was found in five samples，rosiglitazone was found in two samples，tolbutamide was found in one sample，pioglitazone was found in one sample，glidenclamide was found in five samples.