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投稿时间:2018-10-22
投稿时间:2018-10-22
中文摘要: 研究超声波协同微波辅助提取蝉花孢梗束中麦角甾醇的工艺。考察提取时间、乙醇浓度、液料比、超声波功率对麦角甾醇提取得率的影响,然后选用提取时间、液料比、超声波功率3 个因素进行响应面优化试验。结果表明,蝉花孢梗束中麦角甾醇最佳工艺为提取时间15 min,超声波功率120 W,液料比80 ∶1(mL/g),提取溶剂95%乙醇,微波功率100 W。此条件下,麦角甾醇提取得率为2.68 mg/g,与超声波破碎法的麦角甾醇提取得率2.13 mg/g 相比,高出25.82%。
中文关键词: 蝉花 麦角甾醇 超声波协同微波辅助提取 高效液相色谱法 响应面
Abstract:The ultrasonic and microwave assisted extraction technology of ergosterol from synnemata of Isaria cicadae was studied.The effects of extraction time,ethanol concentration,liquid-solid ratio and ultrasonic power on the extraction yield of ergosterol were investigated.And then response surface methodology was performed to optimize the extraction process by using three factors:extraction time,liquid-solid ratio and ultrasonic power.The results indicated that optimal condition was as follows:extraction time 15 min,ultrasonic power 120 W,liquid-solid ratio 80 ∶1 (mL/g),solvent 95%ethanol and microwave power 100 W.Under the optimal conditions,the ergosterol extraction yield was 2.68 mg/g,which was 25.82 % higher than the yield of 2.13 mg/g obtained by the ultrasonic extraction.
keywords: Isaria cicadae ergosterol ultrasonic and microwave-assisted extraction high performance liquid chromatography response surface methodology
文章编号:201919004 中图分类号: 文献标志码:
基金项目:科技部星火计划项目(2015GA710033);滁州学院科研项目(2017PY04);滁州学院大学生创新训练项目(2018CXXL067);滁州学院生物与食品工程学院实验室开放课题(SWSP201514KF)
作者 | 单位 |
许艳红,于士军,陈磊,于爱文,柴新义,王维坤 | 1.滁州学院生物与食品工程学院,安徽滁州239012;2.福建农林大学食品科学学院,福建福州350002;3.通用生物系统安徽有限公司,安徽滁州239000 |
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