Abstract:The anti-alcohol mechanism of sulfated seabuckthorn leaf polysaccharide （SSLP）was explored.Sulfated polysaccharide from seabuckthorn leaf polysaccharide（SLP）was derived by using the chlorosulfonic acid-pyridine method.The content of sulfate radical was determined by barium sulfate turbidity.The polysaccharide esterification was confirmed by infrared spectroscopy.The Valle&Hoch method was used to detect the influence of SSLP and SLP on alcohol dehydrogenase（ADH）activity.Mouse drunk model was established by eating alcohol.The latent time of drunkenness and the durative time of drunkenness were recorded.The activities of ADH in serum were determined.The infrared spectrum（IR）spectra showed that the characteristic absorption peaks of S=O and C-O-S appeared near 1 244.07 cm-1and 808.16 cm-1in SSLP.Compared with the model group，positive group，SLP group and SSLP groups could increase sleep latency significantly and reduce the drunken sleep time significantly，increase the content of ADH markedly and SSLP exhibits stronger anti alcohol effect than SLP.It was indicated that both SSLP and SLP have good anti alcohol effect and SSLP hangover activity was better，so the biological activity of SLP could be enhanced by derivatization.