Abstract:The nutritional value of honey is very rich.Carbohydrate content is the most important ingredient in honey，and carbohydrate accounts for more than 75 % of the total.High performance liquid chromatography coupled with differential refractive detector was used to detect five sugars （fructose，glucose，sucrose，maltose and lactose），in nine different regions of honey and two kinds of adulterated honey，and then using simple cluster analysis to trace the origin of honey，the results showed that the highest sugar content in honey was fructose，followed by glucose and sucrose.The contents of sugars in adulterated honey and true honey of different varieties and different origins were obviously different，which lay a foundation for the research on origin tracing of honeys and identification of adulterated ones.Based on the quantitative results of sugar in honey，the three kinds of honey from different origins had been well distinguished，and the discrimination effect of high fructose comsyrup adulterated honey and real honey was also satisfactory.This method was feasible and effective，and suitable for application.