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投稿时间：2019-04-12
投稿时间：2019-04-12
中文摘要: 以刺梨（Rosa roxburghii Trat）为原料，采用正交试验优化刺梨多酚氧化酶（polyphenol oxidase，PPO）的提取条件，并研究不同抑制剂[抗坏血酸、乙二胺四乙酸二钠（ethylenediaminetetraacetic acid disodium salt，EDTA-2Na）、柠檬酸]对刺梨PPO 活性的影响。结果表明，PPO 的最佳提取工艺条件为：底物邻苯二酚的浓度为0.12 mol/L，提取温度为30 ℃，缓冲液的pH 值为6.0。同时，抗坏血酸、EDTA-2Na、柠檬酸对刺梨PPO 活性均有抑制效果，其中抗坏血酸对PPO 活性抑制效果最强，其次是柠檬酸、EDTA-2Na。
Abstract:Using the fruit of Rosa roxburghii Tratt as raw material，orthogonal experiment was used to optimize the extraction conditions of polyphenol oxidase （PPO）in Rosa roxburghii Tratt，and the effects of different inhibitors [ascorbic acid，ethylenediaminetetraacetic acid disodium salt （EDTA-2Na）and citric acid]on PPO activity in Rosa roxburghii Tratt were studied.The experimental results showed that the optimal extraction conditions of PPO were as follows：the catechol concentration assubstrate 0.12 mol/L，the extraction temperature 30 ℃，and the buffer pH value 6.0.Meanwhile，all the compounds such as ascorbic acid，EDTA-2Na and citric acid，had inhibiting effects on PPO activity in Rosa roxburghii Tratt，among which ascorbic acid had the strongest inhibiting effect on PPO activity，followed by citric acid and EDTA-2Na.
keywords: Rosa roxburghii Tratt polyphenol oxidase inhibitors spectrophotometry process optimization
文章编号：201915014 中图分类号： 文献标志码：
基金项目:贵州大学培育项目（黔科合平台人才[2017]5788-24）
| Author Name | Affiliation |
| YU Lu，MU Bo，JI Sheng-yang | School of Liquor and Food Engineering，Guizhou University，Guiyang 550025，Guizhou，China |
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