Abstract:A quantitative method for the determination of 2-methylimidazole and 4-methylimidazole in yellow wine by ultra-high performance liquid chromatography-tandem mass spectrometry with isotope internal standard dilution was established.The isotope internal standard was added to the sample.The sample was extracted with 1 % trichloroacetic acid，and then purified by the the MCX solid phase extraction column.It was detected by ultra-high performance liquid chromatography-series mass spectrometry and quantified by internal standard method.Under optimal conditions，the detection limits of 2-methylimidazole and 4-methylimidazole were 0.5 μg/kg and 0.05 μg/kg，respectively，the linear relationship was good in the concentration ranges of 0.1 ng/mL～1 000 ng/mL and 1 ng/mL～1 000 ng/mL.The linear correlation coefficient was greater than 0.999.