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食品研究与开发:2019,40(14):20-24
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乳酸菌L-SZ303发酵产γ-氨基丁酸条件的优化
WANG Bing-cong,WU Qiong,CHI Yan-ping
Optimization of Fermentation Conditions for γ-Aminobutyric Acid Production by Lactobacillus L-SZ303
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投稿时间:2018-10-08    
中文摘要: 通过对乳酸菌L-SZ303 发酵产γ-氨基丁酸(γ-aminobutyric acid,GABA)的条件设计了一系列优化试验,以获得最佳的发酵条件。正交试验确定其最适培养基成分为:葡萄糖5 g/L,胰蛋白胨25 g/L,丁二酸钠3 g/L,酵母粉6 g/L,米糠5 g/L,L-谷氨酸钠4 g/L。响应面法确定最佳培养条件为:初始pH6.8,发酵温度36 ℃,发酵时间3 d。优化之后GABA 的产量可达13.375 g/L。
中文关键词: γ-氨基丁酸  乳酸菌  发酵  正交  响应面
Abstract:A series of optimization experiments were designed to obtain the optimal fermentation conditions by fermenting γ-aminobutyric acid (GABA)to lactobacillus L-SZ303.The optimal fermentation medium composition was determined by orthogonal design:5 g/L glucose,25 g/L tryptone,3 g/L sodium succinate,6 g/L yeast powder,5 g/L rice bran,and 4 g/L L-Glu sodium.Then the Box-Behnken design,the optimal culture conditions were initial medium pH6.8 and 36 ℃ for 3 d.After optimization,the yield of GABA can reach 13.375 g/L.
文章编号:201914004     中图分类号:    文献标志码:
基金项目:吉林省科技厅重点科技攻关项目(20170204022NY)
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