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食品研究与开发:2019,40(14):7-12
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微波预处理-超声波辅助提取紫山药多糖及抑制α-葡萄糖苷酶活性研究
WANG Yan-ping,CHEN Yue-ying*,JIA Yan-jie,CAO Ya,XU Shi,LOU Fang-hui
Microwave Pretreatment and Ultrasonic Assistant Extraction of Polysaccharides from Purple Yam and Its Inhibition on α-Glucosinase
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投稿时间:2019-03-14    
中文摘要: 以紫山药粉为原料,对微波预处理-超声波提取紫山药多糖的工艺进行优化,并以α-葡萄糖苷酶抑制模型研究其对α-葡萄糖苷酶活性的抑制作用。通过单因素及正交试验确定最佳提取工艺为料液比1∶40(g/mL)、微波功率300 W、微波时间30 s、超声功率270 W、超声时间30 min。在最佳工艺条件下,紫山药多糖平均得率为11.12%。醇沉后的紫山药多糖粉末中多糖的质量分数为45.80%。α-葡萄糖苷酶活性抑制试验中,紫山药多糖表现出明显的抑制作用,对α-葡萄糖苷酶抑制能力较阿卡波糖弱。
Abstract:Taking purple yam as the experimental material,the extraction technology of polysaccharides from purple yam by microwave pretreatment and ultrasonic extraction was optimized,and the inhibition effect on αglucosinase was studied by using the inhibition model of α-glucosinase.Through single factor and orthogonal test,the optimum condition were as follows:material-solvent ratio 1∶40 (g/mL),microwave power 300 W,microwave time 30 s,ultrasonic power 270 W,ultrasonic time 30 min.Under such conditions,the yield of polysaccharides was 11.12 %.The mass fraction of polysaccharides from purple yam polysaccharides powder was 45.80 % after deproteinization and ethanol precipitation.In the α -gluconidase inhibition test,the polysaccharide from purple yam showed a significant inhibitory effect,and the inhibition ability of α -gluconidase was weaker than that of Aqiabo.
文章编号:201914002     中图分类号:    文献标志码:
基金项目:河南省科技厅科技攻关计划项目(182102110185)
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