Abstract:Orthogonal test method was used to optimize the extraction technology of total flavonoids from Cardiocrinum cathayanum.On the basis of single-factor test，the extraction conditions （ethanol concentration，extraction temperature，ratio of water to materials and extraction times）were analyzed and optimized by the orthogonal design.Meanwhile，the scavenging effects of total flavonoids from Cardiocrinum cathayanum on DPPH·were determined.The results showed that the optimum extraction conditions were as follows：ethanol concentration was 70%，extraction temperature was 60 ℃，ratio of raw material to liquid was 1∶10（g/mL）and extracting for 30 min.Under the conditions，the yield of total flavonoids was 10.90 mg/g.This method was suitable for the extraction of total flavonoids from Cardiocrinum cathayanum with high extraction rate.The total flavonoids from Cardiocrinum cathayanum and vitamin C had the DPPH radical scavenging ability with EC50of 4.439 μg/mL and 11.297 μg/mL，respectively.The results of in vitro antioxidant test showed that the scavenging effects of total flavonoids extract from Cardiocrinum cathayanum to DPPH·was better than that of vitamin C solution at the same concentration.