Abstract:The neuroprotective effect and mechanism of ginsenoside Rg1 on LRRK2 mutation -induced drosophila model of drosophila melanogaster was studied.TH-GAL4；UAS-LRRK2-1915T strain drosophila（abbreviated as 1915T）was used for the experiment.The 1915T strain drosophila was used as the pathological model group，and the TH -GAL4 strain drosophila was used as the negative control group.Different concentrations of ginsenoside Rg1 were administered.After a certain period of time，the pathological modelgroup of parkinson's syndrome （PD）was compared with the control group，and the life cycle was tested by drosophila life test to select the optimal drug concentration.Neuronal apoptosis was further detected by screening the optimal concentration of the drug.At the same time，the protein was extracted from the head of drosophila，and the expression of P-LRRK2，Nrf2，GCLC，P-P38MAPK，ERK，TH and other proteins were detected by Western blot.The optimal drug concentration of ginsenoside Rg1 was determined to be 0.4 mmol/L.Simultaneous treatment of drosophila during larval development and adult development with 0.4 mmol/L ginsenoside Rg1 significantly reduced the survival rate of drosophila，decreased crawling ability and decreased dopamine content caused by LRRK2 mutation.Ginsenoside Rg1 may protect dopaminergic neurons in PD by P-38 signaling pathway to reduce pathological symptoms of PD model drosophila.