本文已被：浏览 1298次 下载 329次
投稿时间：2018-10-03
投稿时间：2018-10-03
中文摘要: 建立测定茶叶中蒽醌的快速气相色谱-串联质谱法（gas chromatography-tandem mass spectrometry，GC-MS/MS）。茶叶样品加入蒽醌-D8 同位素内标应用液后，经乙腈提取，使用QuEChERS 方法净化，氮吹浓缩后，在选择多反应监测模式（multiple reaction monitoring，MRM）下扫描，以 HP-5MS（30 m×250 μm，0.25 μm）色谱柱进行分离，内标法定量。蒽醌在10 ng/mL～200 ng/mL 范围内线性关系良好，相关系数（r=0.999 5）。该方法的检出限为3 μg/kg，定量限为10 μg/kg，加标回收率在87.1%～98.4%之间，加标回收试验相对标准偏差（relative standard deviation，RSD）在1.23%～4.12%之间。该方法适用于茶叶中蒽醌的定性定量检测。
中文关键词: 茶叶 蒽醌 气相色谱-串联质谱法 净化 内标法
Abstract:To establish a rapid determination method of anthraquinone in tea by gas chromatography-tandem mass spectrometry （GC-MS/MS），sample added with anthraquinone-D8 isotope internal standard were extracted by acetonitrile.QuEChERS method was used to purify extracts.Sample was separated by HP-5 MS（30 m×250 μm，0.25 μm），detected by select multiple reaction monitoring （MRM）mode.Internal standard method was used as quantitative.It had a good linear relationship in the experimental concentration range of 10 ng/mL-200 ng/mL，and the correlation coefficient was 0.999 5.The detection limit of this method was 3 μg/kg，the quantitative limit was 10 μg/kg.The recoveries of anthraquinone were bet-ween 87.1%-98.4%，the RSD was between 1.23%and 4.12%.The method was suitable for qualitative and quantitative determination of anthraquinone in tea.
keywords: tea anthraquinone gas chromatography-tandem mass spectrometry purifying internal standard method
文章编号：201912037 中图分类号： 文献标志码：
基金项目:山东省医药卫生科技发展计划项目（2017WS378）；淄博市科学技术发展计划项目（2017kj010071）
引用文本：
津ICP备20000603号-1