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投稿时间：2019-02-21
投稿时间：2019-02-21
中文摘要: 以大豆11S 球蛋白为原料，通过胰蛋白酶水解制备活性肽，在单因素试验的基础上，采用响应面法确定最佳酶解条件，并检测大豆肽溶液的体外抗氧化性。结果表明，底物浓度2 mg/mL 时的最佳水解条件为：酶和底物比4 900 U/g，pH8.1，温度44℃，水解时间2.6 h，此条件下经验证试验得到大豆肽的水解度为89.25%。大豆肽溶液对超氧阴离子自由基（O2-·）和羟自由基（·OH）都具有良好的清除效果，随着大豆肽溶液浓度的增加，O2-·和·OH 的清除率也随之增加。当肽液浓度为12 mg/mL 时，对O2-·的清除率最高达到46.86%；当肽液浓度为0.5 mg/mL 时对·OH 的清除率最高，达到33.73%。
Abstract:Soybean 11S globulin was used as raw material to prepare active peptide by trypsinase hydrolysis.On the basis of single factor experiment，response surface methodology was used to determine the optimal enzymatic hydrolysis conditions，and test the in vitro antioxidant activity of soy peptide solution.The results showed that the optimum hydrolysis conditions were as follows：the ratio of enzyme to substrate was 4 900 U/g，the pH was 8.1，the temperature was 44℃，the hydrolysis time was 2.6 h，the degree of hydrolysis of the soybean peptide obtained by the verification test under this condition was 89.25 %.Soybean peptide solution had good scavenging effects on superoxide oxygen free radical（O2-·）and hydroxyl radical（·OH），and the scavenging rates of O2-·and·OH increased with the increase of concentration of soybean peptide solution.When the concentration of peptide solution was 12 mg/mL，the clearance rate of O2-·reached 46.86 %，and when the concentration of peptide solution was 0.5 mg/mL，the clearance rate of·OH reached 33.73%.
文章编号：201911016 中图分类号： 文献标志码：
基金项目:吉林省教育厅“十三五”科学技术研究项目（2016296）
| Author Name | Affiliation |
| MA Fu-jian，GAO Chang-cheng，XIE Di，LIU Ya-nan，ZOU Xian-feng* | College of Food Science and Engineering，Changchun University，Changchun 130022，Jilin，China |
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