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投稿时间：2018-12-11
投稿时间：2018-12-11
中文摘要: 采用酶活性测定法及气相色谱-质谱法对猕猴桃根石油醚提取物的α-葡萄糖苷酶抑制作用及组成成分进行研究。酶活性结果表明，提取物对α-葡萄糖苷酶在最大考察浓度的抑制率可达（89.38±1.27）%，优于同浓度下的阿卡波糖。气相色谱-质谱法分析结果表明，从提取物的甲酯化和非甲酯化样品中共检测鉴定出49 种成分，甲酯化样品中检测鉴定了20 种，非甲酯化样品中检测鉴定了29 种，其中亚油酸乙酯、油酸乙酯、棕榈酸乙酯的相对含量总和占流出峰总面积的61.484%。猕猴桃根石油醚提取物含有的亚油酸、亚油酸甲酯、亚油酸乙酯可能是猕猴桃根石油醚提取物抑制α-葡萄糖苷酶的主要物质。
Abstract:The inhibition of α-glucosidase of petroleum ether extracts from Actinidia chinensis radix were studied by the method of enzymatic activity determination.Subsequently，the chemical constituent of the extracts was analyzed by gas chromatography-mass spectrometry.The results showed that the inhibition of αglucosidase was （89.38 ± 1.27）% at the maximum concentration.The results of gas chromatography-mass spectrometry showed that 49 compounds of the petroleum ether extracts were identified，including 29 components from the non-methylated samples and 20 components from the methylated samples.The total relative content of ethyl linoleate，ethyl oleate and ethyl palmitate accounts for 61.484 % of the total outflow peak area in non-methylated samples.In summary，it was assumed that linoleic acid，methyl linoleate and ethyl linoleate were the substances that inhibit α-glucosidase from Actinidia chinensis radix petroleum ether extracts.
keywords: Actinidia chinensis radix petroleum ether extraction α-glucosidase gas chromatography-mass spectrometry（GC-MS） constituent analysis
文章编号：201909026 中图分类号： 文献标志码：
基金项目:贵州省科技计划项目（黔科合LH 字[2017]7302 号）；遵义市科技局人才团队项目（遵市科合社字[2018]36 号）
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