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食品研究与开发:2019,40(9):13-17
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胰蛋白酶酶解制备卵转铁蛋白源抗菌活性肽条件的研究
(1.天津商业大学生物技术与食品科学学院,天津市食品生物技术重点实验室,天津300134;2.天津市食品研究所有限公司,天津301609;3.天津大学化工学院,天津300072)
Preparation of Antimicrobial Peptide from Ovotransferrin with Trypsin
(1.College of Biotechnology and Food Science,Tianjin University of Commerce,Tianjin Key Laboratory of Food Biotechnology,Tianjin 300134,China;2.Tianjin Food Research Institute Co.,Ltd.,Tianjin 301609,China;3.School of Chemical Engineering and Technology of Tianjin University,Tianjin 300072,China)
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投稿时间:2018-10-11    
中文摘要: 卵转铁蛋白具广谱抗菌性,现已成为生物医学领域研究的热点。以"两步醇析法"获得的卵转铁蛋白为研究对象,经胰蛋白酶酶解制备抗菌活性肽,优化酶解条件,并研究其对大肠杆菌的抗菌作用。结果表明,制备卵转铁蛋白抗菌肽抑菌效果最好的试验条件为:pH 值8.5,温度55 ℃,底物浓度30 mg/mL,酶与底物浓度比1∶300(U/mg),酶解时间1.5 h,在该条件下进行酶解,得到的抗菌肽抑菌率为89.152%。
中文关键词: 卵转铁蛋白  抗菌肽  胰蛋白酶  酶解  抑菌率
Abstract:Ovotransferrin has become a hot topic in biomedical research because its broad spectrum of antibacterial activity.The ovotransferrin obtained from the "two step alcohol-precipitation" was studied.The antibacterial peptides were prepared by the enzymolysis technique-trypsin,and the hydrolysis conditions were optimized to study the antibacterial rate of E.coli.The results showed that the best experimental conditions were as follows:pH8.5,55 ℃,substrate concentration 30 mg/mL,enzyme bottom ratio 1∶300(U/mg),enzyme hydrolysis time 1.5 h.The best bacteriostasis rate was 89.152%.
文章编号:201909003     中图分类号:    文献标志码:
基金项目:天津市应用基础与前沿技术研究计划青年项目(15JCQNJC12200)
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