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食品研究与开发:2019,40(9):1-7
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菌酶协同发酵水解大米蛋白ACE抑制肽及其活性的研究
(1.中国海洋大学基础教学中心,山东青岛266003;2.哈尔滨工业大学化工与化学学院,黑龙江哈尔滨150090;3.哈尔滨市食品工业研究所有限公司,黑龙江哈尔滨150090;4.中恩(天津)营养科技有限公司,天津300000;5.华羚生物技术研究中心,甘肃兰州730000)
Lactobacillus plantarum and Bacillus subtilis Coupled with Enzymes to Remove Rice Protein and the Angiotensin I-Converting Enzyme Inhibitory Activities of Removal Liquid
(1.Teaching Center of Fundamental Courses,Ocean University of China,Qingdao 266003,Shandong,China;2.School of Chemistry and Chemical Engineering,Harbin Institute of Technology,Harbin 150090,Heilongjiang,China;3.Harbin Food Industry Research Institute Co.,Ltd.,Harbin 150090,Heilongjiang,China;4.Sinofn(Tianjin)Nutrition Science and Technology Co.,Ltd.,Tianjin 300000,China;5.Hua Ling Biotechnology Research Center,Lanzhou 730000,Gansu,China)
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投稿时间:2018-09-28    
中文摘要: 使用植物乳杆菌2-18 和枯草芽孢杆菌Y、枯草芽孢杆菌Y4-2 联合蛋白酶共同水解大米蛋白,测定大米蛋白的水解溶出率及脱除液的血管紧张素转换酶(angiotensin I-converting enzyme,ACE)抑制活性。在利用菌酶联合水解大米蛋白的组合中,植物乳杆菌2-18+风味蛋白酶/菠萝蛋白酶+胃蛋白酶/酸性蛋白酶组合对大米蛋白的水解溶出率最高,蛋白脱除率为(91.32±1.60)%。大米蛋白水解溶出液中必需氨基酸占总氨基酸含量的39.62%。通过对分子量分布分析,大米蛋白水解溶出液的多肽分子量分布主要在1 kDa ~1.5 kDa 部分。经植物乳杆菌2-18+风味蛋白酶/菠萝蛋白酶+胃蛋白酶/酸性蛋白酶组合对大米蛋白的水解溶出液ACE 抑制率达(91.95±1.63)%,具有良好的ACE 抑制活性。
Abstract:The rice protein was removed by the combination of Lactobacillus plantarum 2-18 and Bacillus subtilis Y, Bacillus subtilis Y4-2,and the rice protein removal rate and the angiotensin I-converting enzyme(ACE)inhibitory activity of rice protein removal solution were determined.In the groups of using the combination of bacterium and enzyme to remove the rice protein,the combination of Lactobacillus plantarum 2-18+ Flavor protease/bromelain+pepsin/acid protease had the highest removal rate of rice protein,which was(91.32±1.60)%.The essential amino acids in the rice protein removal solution accounted for 39.62 % of the total amino acid content.Through molecular weight distribution analysis,the molecular weight distribution of the peptide of the rice protein removal solution is mainly in the 1 kDa -1.5 kDa portion.The ACE inhibition rate of the protein removal solution obtained by Lactobacillus plantarum 2-18+ flavor protease/bromelain + pepsin/acid protease combined with rice protein was(91.95±1.63)%,and its removal solution has well ACE inhibitory activity.
文章编号:201909001     中图分类号:    文献标志码:
基金项目:国家重点研发计划(2018YFC1604302);甘肃省科技重大专项计划项目(17ZD2NP025);中恩(天津)营养科技有限公司与中国海洋大学技术开发项目
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