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投稿时间:2018-09-27
投稿时间:2018-09-27
中文摘要: 采用水提法对红茶、乌龙茶、绿茶和茉莉花茶进行提取,并对抽提物中总酚含量和各个儿茶素单体含量进行测定,通过常见的3 种抗氧化能力测试方法,即DPPH 法、2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐[(2,2-azino-bis (3-ethyl-benzothiazoline-6-sulfonic acid)diammonium salt,ABTS)]法及铁离子还原/抗氧化能力(ferric ion reducing antioxidant power,FRAP)法对4种茶叶水提物的体外抗氧化能力进行评价,同时比较茶多酚和VC 的抗氧化活性。结果表明:4种茶叶水提物均有很强的体外抗氧化活性,绿茶、茉莉花茶、乌龙茶的DPPH 法及FRAP 法抗氧化活性测试结果优于红茶,红茶的ABTS+·清除率略高于其他3 种茶。相关性分析结果显示儿茶素总量、总酚含量、没食子儿茶素(gallocatechin,GC)、表没食子儿茶素(epigallocatechin,EGC)、表没食子儿茶素没食子酸酯(epigallocatechin gallate,EGCG)、表儿茶素没食子酸酯(epicatechin gallate,ECG)含量和DPPH 法、FRAP 法结果呈现正相关,而与ABTS 法结果呈现负相关。此外,试验结果表明茶多酚的抗氧化能力高于VC。
Abstract:Hot water extraction technique was used for the preparation of four kinds of tea infusions and four tea water extracts were analyzed for their total phenolic content and catechins.Antioxidant activities were evaluated using three most common methods,i.e. DPPH radical scavenging assay,ferric reducing antioxidant power(FRAP)and 2,2-azino-bis (3-ethyl-benzothiazoline-6-sulfonic acid)diammonium salt(ABTS)cation radical scavenging assay. Meanwhile,the antioxidant capacity between tea polyphenol and VC was also evaluated. Results showed that four tea water extracts had strong antioxidant activity. Green tea,jasmine tea,and oolong tea had higher values on DPPH and FRAP assays than black tea.Black tea exhibited slightly higher ABTS cation radical scavenging activity than the other three teas. Correlation analysis showed that there was a significant positive correlation between the levels of total catechins,total phenolic content,gallocatechin(GC),epigallocatechin(EGC),epigallocatechin gallate(EGCG),epicatechin gallate(ECG)and antioxidant activities measured using the DPPH and FRAP assays,however a negative correlation using ABTS assay.In addition,our result showed that tea polyphenols had stronger antioxidant potential than VC.
文章编号:201908011 中图分类号: 文献标志码:
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