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投稿时间:2018-09-07
投稿时间:2018-09-07
中文摘要: 利用低大米蛋白制备过程发酵水解液中富含大米蛋白和肽。以乳杆菌L2-18+风味蛋白酶/菠萝蛋白酶+胃蛋白酶/酸性蛋白酶分步获得的大米蛋白水解液为研究对象,通过超滤获得小于3 kDa 蛋白肽。经阳离子交换、凝胶柱层析和高效液相色谱(high performance liquid chromatography,HPLC)三步分离纯化,确定出高活性ACE抑制肽组分,IC50值为33.81 μg/mL。经液质联用(liquid chromatograph-mass spectrometer,LC-MS)鉴定分析,其多肽序列为VVFFAAAL。
Abstract:Removal solution for the preparation of low protein rice for kidney disease patients is rich in rice protein and peptides. This paper mainly investigated the step-by-step removal of rice by Lactobacillus plantarum 2-18+Flavor protease/bromelain+pepsin/acid proteasen and the removal solution less than 3 kDa was obtained by ultrafiltration.The protein removal solution was separated and purified by cation exchange,gel column chromatography and high performance liquid chromatography(HPLC)three-step separation and the highest ACE inhibitory activity components in the removal solution was identified by liquid chromatograph-mass spectrometer(LC-MS).The peptide sequence was VVFFAAAL,which IC50 value was 33.81 μg/mL.
keywords: rice protein combination of Lactobacillus and enzymes ACE inhibitory activity separation and purification amino acid sequenceZOU Junzhe,LIN Kai,YANG Xu ,et al. Lactobacillus plantarum Coupled with Proteinases to Remove Rice Protein for the Production of ACE Inhibitory Peptides[J].Food Research and Development,2019,40(8):1-6
文章编号:201908001 中图分类号: 文献标志码:
基金项目:国家重点研发项目(2018YFC1604302);甘肃省科技重大专项(17ZD2NP025);中恩(天津)营养科技有限公司与中国海洋大学技术开发项目
作者 | 单位 |
邹俊哲,林凯,杨旭,谯飞,韩雪,刘红彦,杨艳艳,刘汉民,张兰威 | 1.中国海洋大学基础教学中心,山东青岛266003;2.哈尔滨工业大学化工与化学学院,黑龙江哈尔滨150090;3.哈尔滨市食品工业研究所有限公司,黑龙江哈尔滨150090;4.中恩天津营养科技有限公司,天津300000;5.华羚生物技术研究中心,甘肃兰州730000 |
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