Abstract:The paper aimed to mouse feeding study，investigate the effect of fresh betel nut three different ways to eat on the growth and immune function in mice，provide a reference for the safety evaluation of betel nut.Betel fresh food prepared by three different ways aqueous extract，mice fed regular meals，and recording the growth characteristics mice immune indices determined periodically.The results showed that：the consumption of fresh betel three different ways exist on body weight in mice affected to different degrees，wherein the fresh food group betel embodiment could significantly inhibit the mouse body weight gain （p＜0.05），group betel and tobacco could also be different groups of inhibition of mouse weight gain.For the organ index，the temporal lobe group had the most significant effect on the liver organ index （p＜0.05）.The fresh betel nut group had the most significant effect on the spleen organ index （p＜0.05），and the red blood cell count was three different.There was no significant difference in the way of eating.However，in terms of white blood cell count，the white blood cell counts of the cut tobacco group and the temporal lobe group were significantly higher than those of the fresh betel nut group and the control group.For cytokines and immune globulins，tumor necrosis factor tobacco content was significantly higher and fresh betel group （p＜0.05），was significantly higher compared to the cytokine interleukin-1 content of the control group （p＜0.05），immunoglobulin A and immunoglobulin G was significantly lower（p＜0.05）.For cytokines and immunoglobulin，the content of tumor necrosis factor in tobacco group was significantly higher than that in control group and fresh betel nut group （p＜ 0.05），the content of cytokine interleukin-1 was significantly higher than that in control group （p＜0.05），and the content of immunoglobulin M in tobacco group was significantly higher than that in control group （p＜0.05）.There were no differences in the remaining groups.It could be seen that the different eating modes of the three kinds of fresh betel nut have different negative effects on the body weight and immune function of the mice.The negative effects of the eating mode of the tobacco and the betel leaf group on the immune function of the mice were more obvious.