Abstract:Lactoferrin is a kind of active glycoprotein with great nutritional value，which is widely used in infant milk powder.At present，there is no relevant standard for the determination of lactoferrin at home and abroad.Heparin affinity column（HiTrapTMHeparin HP column）or equivalent were applied to the purification process of lactoferrin before pretreatment.An efficient and convenient method for the determination of lactoferrin in foods was established.The lactoferrin in the sample was extracted by Na2HPO4buffer solution（0.20 mol/L，pH=8.00±0.5）.The extract was purified by heparin affinity column，washed with Na2HPO4buffer solution，and Na2HPO4-NaCl buffer solution was eluted and the final volume to 3.0 mL.High performance liquid chromatography（HPLC）was used with ultraviolet absorption detector（UV）or diode array detector（DAD）at 280 nm，and the external standard method was quantified.It was the first time to put forward the idea that heparin affinity column was applied to the purification of lactoferrin before detection.The improved method was suitable for detection of lactoferrin in detection mechanism or dairy enterprises.